ISSN 2360-7955
Abstract
Accepted 1st December, 2014
Antibiotic resistance has been recognized as a worldwide emerging problem in both human and animals. To investigate the prevalence and antibiotic susceptibility pattern of E.coli among the birds at Madonna University poultry, fresh droppings were streaked on MacConkey and EMB agar plates and incubated for 24h at 37ºC. Emergent colonies were identified and characterized as E.coli by using a standard bacteriological techniques and serotype i.e. E. coli 0157 Latex agglutination. Disk diffusion method was used to evaluate the susceptibility of the isolates against 8 antibiotics. Among the samples collected from 100 birds (25% old layers, 30% new layers, 25% main layers and 20% cockrells), E.coli was isolated from 95 comprising 21.1% old layers, 31.6% new layers, 24.3% main layers and 21.1% broilers. All the tested strains were found resistant to tetracycline, nalidixic acid, chloramphenicol, co-trimaoxazole, ceftriazole, cefpirome and cefoxitin while 10% were susceptible to ciprofloxacin. The distribution of E.coli 0157:H7 showed 10%, 20%, 33.3% and 30% occurrence in old layers, new layers, main layers and broilers respectively. This abnormal antibiotics resistance observed in this study indicates that poultry is a major source of antibiotics resistance traits.
Keywords: E. coli, poultry, multidrug resistance.