International Journal of Bacteriology, Virology and Immunology

Investigation of Biofield Treated Vitamin D3 to Improve Immunity of Bone Cells in MG-63 Cell Line.

Abstract

Accepted 29^th June, 2018.

 

The study was aimed to evaluate the effect of Consciousness Energy Healing-based vitamin D₃ and DMEM on bone health in human bone osteosarcoma cells (MG-63). Test items (vitamin D₃ and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Inthirani Arul and those samples were denoted as Biofield Treated (BT) samples, while other parts of each sample were referred as untreated test items (UT). Cell viability revealed that test samples were found as safe in tested concentrations. ALP was significantly increased by 260% in BT-DMEM + BT-TI group at 0.1 µg/mL compared to UT-DMEM + UT-TI group. Moreover, collagen level was significantly increased by 102.94% and 52.94% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 0.1µg/mL than UT-DMEM + UT-TI group. Further, collagen level was significantly increased by 114.94%, 114.03%, and 152.33% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 1µg/mL than untreated. Besides, percent of bone mineralization was significantly enhanced by 82.95% and 119.55% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 10µg/mL than untreated. Moreover, it was significantly increased by 166.44% and 212.49% in BT-DMEM + UT-TI and BT-DMEM + BT-TI groups, respectively at 50µg/mL than untreated. It was also significantly increased by 104.44%, 100%, and 230.22% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 100µg/mL than untreated. Among three combination regimens, the Biofield Treated vitamin D₃ and DMEM group was comparatively more improved the bone-related parameters and might be useful against various bone-related disorders (rickets, low bone density, osteomalacia, osteoporosis, Paget’s disease, chondrodystrophia fetalis), stress management, anti-aging, autoimmune and inflammatory disorders near future.

 

 

Keywords: The Trivedi Effect^®, Bone health, Biofield Energy Healing Treatment, Vitamin D₃, Osteosarcoma cells

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Sambhu Charan Mondal2 and Snehasis Jana2* on Google Scholar Sambhu Charan Mondal2 and Snehasis Jana2* on Pubmed Mahendra Kumar Trivedi1 on Google Scholar Mahendra Kumar Trivedi1 on Pubmed Alice Branton1 on Google Scholar Alice Branton1 on Pubmed Dahryn Trivedi1 on Google Scholar Dahryn Trivedi1 on Pubmed Gopal Nayak1 on Google Scholar Gopal Nayak1 on Pubmed Inthirani Arul1 on Google Scholar Inthirani Arul1 on Pubmed