Heavy metals are of particular importance from the agricultural and eco-toxicological point of view. Information on their content in plant and animal is of great botanical, nutritional and environmental interest. This study was designed, to investigate the effect of copper on Chlorophyll a, Chlorophyll b and total chlorophyll contents and results were correlated with results of accumulation of intracellular copper estimated by atomic absorption spectroscopy (AAS), in leaves of Brassica juncea (L.) Czern. Seedlings were treated with copper at 0.5, 1.0, 5.0, 10.0, 20.0, 40.0, 50.0, 75.0 and 100.0 μmol concentrations on day 5 and 10. The varying trends were observed for Chl a, Chl b and total chlorophyll content with respect to corresponding accumulated intracellular copper.


Keywords:  Brassica juncea (L.) Czern., Chlorophyll, Intracellular copper, Scavenging activity, Seedlings.