International Journal of Bacteriology, Virology and Immunology

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Effect of the Biofield Energy Treated Test Formulation on Pro-Inflammatory Cytokines Expression in Mouse Splenocytes

Mahendra Kumar Trivedi and Snehasis Jana




Accepted 1st June, 2019.


A new proprietary formulation was designed with a mixture of the nanocurcumin along with minerals and vitamins viz. zinc chloride, magnesium gluconate, sodium selenate, ascorbic acid (vitamin C), cholecalciferol (vitamin D3), iron sulfate, and copper chloride. The study aimed to evaluate the immunomodulatory activity of Biofield Energy Healing (The Trivedi Effect®) Treated test formulationby the estimation of cytokines in mice splenocyte cells. The test formulation was divided into two different parts, one part was control without any Biofield Energy Treatment, while the other part was defined as Biofield Energy Treated sample, which received Biofield Energy Healing Treatment by Mr. Mahendra Kumar Trivedi. The Biofield Energy Treated test formulation was evaluated to find the expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF-α), macrophage inflammatory protein (MIP-1α), and interleukin (IL-1β) in splenocyte cell culture supernatant along with determination of non-cytotoxic concentrations (MTT assay). MTT assay showed significant cell viability at all the tested concentrations and found safe with more than 88% cell viability.  TNF-α was significantly reduced by 15.21% and 16.41% in the Biofield Energy Treated test formulation group at 0.5 and 5.2µg/mL, respectively as compared with the vehicle control group. The level of IL-1β was also significantly reduced by 13.49%, 13.82%, and 20.44% at 0.1, 8, and 10 µg/mL, respectively as compared to the vehicle control group. Overall, The Trivedi Effect®-Energy of Consciousness Energy Healing Treatment significantly down-regulated the pro-inflammatory cytokines and further potentiate the immunomodulatory effect of the test formulation, which could be better to use against many organ transplantation (for example kidney transplants, liver transplants and heart transplants) and various disease conditions such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease, stress etc. with a safe therapeutic index to improve overall health and quality of life. 


Keywords: The Trivedi Effect®, Biofield Energy Healing Treatment, Pro-inflammatory cytokines, MIP-1α, TNF-α, IL-1β.