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Nickel (II) bio recovery from industrial wastewater by Azotobacterial vineland during Two stage processes
T.A.A. El-barbary 1, M.A El-Badry 2
Abstract
Accepted 1st March, 2019
The ability of heavy metal removal, especially nickel (II) by five different bacterial species was evaluated during this work. Industrial plating waste water contains high concentrations of heavy metals that may contaminate food chain during cultivation. Washing water using industrial water without pre-treatment causes sever health weakness and mutagenesis in many ecosystem living. Nickel (II) is discarded during wastewater each year. Nickel (II) bio recovery as less expensive and environmentally friendly process were screened during this work by five different bacterial species Bacillus megaterium EMCC 1013, Rhizobium rhizogenes EMCC1743, Rhizobium leguminosarum EMCC1130, Azotobacter vinelandii and Nocardiopsis Dassenvillei Azotobacter vinelandii exhibited maximum recovery of Nickel (II) at concentration 10 ppm with 88 % recovery during two stages recovery processes and after optimization condition 24 h with inoculum size 0.1 x 10 29 cfu at PH 7 and energy source glucose and ammonium oxalate as carbon and nitrogen source and 30o C as incubation temprature. The principle target of this work was confirming the bio recovery of Nickel (II) from industrial waste water through two stages to raise the percentage of Nickel (II) removal. In addition, evaluation the bio recovery ability of Nickel (II) by different five microorganisms to utilize them in further studies in the removal of Nickel (II) from plating waste water. Azotobacter vinelandii as the most potent Nickel (II) removal in this study will be used in biotechnology for the bio recovery of Nickel (II) and also used as biomarkers for the detection of Nickel (II)
Keywords: Nickel, Azotobacter vinelandii, Waste water, Heavy metal, bioremoval